ABSTRACT
<p><b>OBJECTIVE</b>To examine the effects of Han's acupoint and nerve stimulator (HANS) electroacupuncture on the expression of NPY in periaqueductal grey (PAG) of heroin addicted rats.</p><p><b>METHODS</b>Heroin was injected subcutaneously according to the principle of daily increasing dose in rats of experimented group. The ability of special learning and memory were tested by Morris water maze; The expression of NPY in PAG of rat were detected by immunohistochemistry.</p><p><b>RESULTS</b>(1) Escape latency and searching distance in heroin-addiction group were significantly increased compared with those of normal group during the place navigation test (P < 0.05). However, in acupuncture group, escape latency and searching distance was obviously shortened compared with those of heroin-addiction group (P < 0.05). The exploring time and distance of original platform area in proportion to the total distance in heroin-addiction group significantly decreased compared with those of normal group during spatial probe test (P < 0.05). The exploring time and distance of original platform area in proportion to the total distance in acupuncture group was increased compared with those in heroin-addiction group (P < 0.01). (2) The expression of NPY of heroin-addiction group was lower than that in normal group in PAG, while those of acupuncture group was higher than that in the heroin-addiction group (P < 0.05).</p><p><b>CONCLUSION</b>The learning and memory induced by heroin-addiction could be reversed and the expression of NPY in PAG was increased by HANS in rats.</p>
Subject(s)
Animals , Male , Rats , Electroacupuncture , Heroin Dependence , Metabolism , Maze Learning , Memory , Neuropeptide Y , Metabolism , Periaqueductal Gray , Metabolism , Rats, WistarABSTRACT
The authors explained the process of organizing and implementing of the designed experiments.The designed experi- ments can improve students' creative thinking ability and activate students'initiative and meanwhile,it can improve teachers' level and contribute to making progress in teaching and studying although there are still some problems to solve.